DMTF1 Antibody / Cyclin-D-binding Myb-like transcription factor 1 Antibody (FY13594)

DMTF1 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using Cyclin-D-binding Myb-like transcription factor 1 Antibody shows brown staining across malignant epithelial structures and associated tumor cells. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.

DMTF1 Antibody by WB in human and rodent samples. Western blot analysis using Cyclin-D-binding Myb-like transcription factor 1 Antibody detects an immunoreactive band near 90 kDa in human K562 (lane 1), SH-SY5Y (lane 2), SiHa (lane 3), U251 (lane 4), rat testis (lane 5), rat thymus (lane 6), mouse testis (lane 7), and mouse thymus (lane 8). The calculated molecular weight is approximately 84.5 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

DMTF1 Antibody by IHC in human stomach carcinoma. Immunohistochemistry of paraffin-embedded human stomach carcinoma using Cyclin-D-binding Myb-like transcription factor 1 Antibody shows brown staining around gland-forming epithelial structures. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.

DMTF1 Antibody by IHC in human breast carcinoma. Immunohistochemistry of paraffin-embedded human breast carcinoma using Cyclin-D-binding Myb-like transcription factor 1 Antibody shows brown staining in tumor cells within irregular glandular structures. Tissue sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection. Hematoxylin provides the blue nuclear counterstain.