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Home >> Antibodies >> DMD Antibody / Dystrophin

DMD Antibody / Dystrophin [clone DMD/3676] (V8862)

  Catalog No Formulation Size Price (USD)  
Image V8862-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8862-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8862SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC staining of FFPE human skeletal muscle tissue with DMD antibody (clone DMD/3676). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human heart muscle tissue with DMD antibody (clone DMD/3676). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free DMD antibody (clone DMD/3676) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using DMD antibody (clone DMD/3676). These results demonstrate the foremost specificity of the DMD/3676 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name DMD/3676
Purity Protein A/G affinity
UniProt P11532
Localization Cell surface, Cytoplasm
Applications ELISA (order BSA-free format for coating) :
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This DMD antibody is available for research use only.
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Description

Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002% of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Application Notes

Optimal dilution of the DMD antibody should be determined by the researcher.

Immunogen

A portion of amino acids 114-263 from the human protein was used as the immunogen for the DMD antibody.

Storage

Aliquot the DMD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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