DLST Antibody / Dihydrolipoamide Succinyltransferase Antibody (FY13511)

DLST Antibody by IHC in human colon cancer. Immunohistochemistry of FFPE human colon cancer tissue using DLST Antibody at 2 ug/ml. Brown DAB immunoreactivity is detected in the tissue section, with hematoxylin counterstaining highlighting cellular and glandular architecture. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Dihydrolipoamide Succinyltransferase Antibody detects the mitochondrial E2 component of the alpha-ketoglutarate dehydrogenase complex.

DLST Antibody by IHC in human lung cancer. Immunohistochemistry of FFPE human lung cancer tissue using DLST Antibody at 2 ug/ml. Brown DAB immunoreactivity is detected in the tissue section, demonstrating DLST expression. Hematoxylin counterstaining highlights nuclei and surrounding tissue architecture. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Dihydrolipoamide Succinyltransferase Antibody recognizes a mitochondrial enzyme involved in cellular energy metabolism.

DLST Antibody by IHC in human appendix tissue. Immunohistochemistry of FFPE human appendix tissue using DLST Antibody at 2 ug/ml. Brown DAB immunoreactivity is detected in the tissue section, with hematoxylin counterstaining highlighting nuclei and tissue architecture. Heat-induced epitope retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This Dihydrolipoamide Succinyltransferase Antibody recognizes a mitochondrial enzyme involved in oxidative metabolism.

DLST Antibody by IF/ in human T47D cells. Immunofluorescence staining of human T47D cells using DLST Antibody at 5 ug/ml. Green fluorescence indicates DLST immunoreactivity, while nuclei are counterstained blue with DAPI. Enzymatic antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was used at 1:500. This Dihydrolipoamide Succinyltransferase Antibody detects a mitochondrial enzyme involved in the TCA cycle.

DLST Antibody by WB in human cells and placenta tissue. Western blot analysis using DLST Antibody at 0.5 ug/ml. Lanes 1-7: human HeLa, HepG2, MCF7, HEK293, T47D, RT4, and U87 cell lysates; lane 8: human placenta tissue lysate. Samples were separated by 10% SDS-PAGE under reducing conditions, with 30 ug of protein loaded per lane. Immunoreactive bands are detected at approximately 43 and 49 kDa, consistent with the reported migration range of DLST. This Dihydrolipoamide Succinyltransferase Antibody recognizes the E2 component of the mitochondrial alpha-ketoglutarate dehydrogenase complex.

DLST Antibody by WB in rat and mouse liver and brain tissue. Western blot analysis of rat liver (lane 1), rat brain (lane 2), mouse liver (lane 3), and mouse brain (lane 4) lysates using DLST Antibody at 0.5 ug/ml. Samples were separated by 10% SDS-PAGE under reducing conditions, with 30 ug of protein loaded per lane. Immunoreactive bands are detected at approximately 43 and 49 kDa, consistent with the reported molecular weight range of DLST. This Dihydrolipoamide Succinyltransferase Antibody detects a mitochondrial enzyme involved in the TCA cycle and cellular energy metabolism.