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Home >> Antibodies >> DLL1 Antibody

DLL1 Antibody (RQ5690)

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  Catalog No Formulation Size Price (USD)  
Image RQ5690 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with DLL1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with DLL1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placenta with DLL1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human ThP-1 cells with DLL1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DLL1 antibody.
Western blot testing of 1) human HeLa, 2) monkey COS-7 and human 3) Jurkat, 4) Raji, 5) SW620, 6) HepG2, 7) U-2 OS, 8) rat heart, 9) rat C6, 10) mouse heart, 11) mouse Neuro-2a, 12) mouse RAW264.7 lysate with DLL1 antibody. Predicted molecular weight ~78 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O00548
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This DLL1 antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human, Mouse, Rat

Description

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. This gene is mapped to chromosome 6q27.

Application Notes

Optimal dilution of the DLL1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q18-K239) was used as the immunogen for the DLL1 antibody.

Storage

After reconstitution, the DLL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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