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Home >> Antibodies >> DIS3 Antibody / RRP44

DIS3 Antibody / RRP44 (RQ4644)

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  Catalog No Formulation Size Price (USD)  
Image RQ4644 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) HEK293, 3) MDA-MB-231 and 4) Caco-2 lysate with DIS3 antibody at 0.5ug/ml. Predicted molecular weight ~109 kDa.
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Western blot testing of human 1) HeLa, 2) HEK293, 3) MDA-MB-231 and 4) Caco-2 lysate with DIS3 antibody at 0.5ug/ml. Predicted molecular weight ~109 kDa.
IHC staining of FFPE human placenta with DIS3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney with DIS3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with DIS3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with DIS3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9Y2L1
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This DIS3 antibody is available for research use only.
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Description

Exosome complex exonuclease RRP44, or Dis3, is an enzyme that in humans is encoded by the DIS3 gene. Its protein product is an RNase enzyme homologous to the yeast protein Rrp44, and can be part of the exosome complex in the nucleus of eukaryotic cells. By genomic sequence analysis, this gene is mapped to chromosome 13q21-q22. Expression of human DIS3 could partially complement Dis3 depletion in yeast.

Application Notes

Optimal dilution of the DIS3 antibody should be determined by the researcher.

Immunogen

Amino acids Q62-A304 from the human protein were used as the immunogen for the DIS3 antibody.

Storage

After reconstitution, the DIS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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