DIRAS3 Antibody / GTP-binding protein Di-Ras3 Antibody (FY13588)

DIRAS3 Antibody by IHC in mouse fallopian tube. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse fallopian tube using GTP-binding protein Di-Ras3 Antibody shows prominent brown staining along portions of the luminal epithelial lining, with blue nuclear counterstaining. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0, followed by blocking with 10% goat serum. Sections were incubated with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen generated brown immunoreactivity, with hematoxylin counterstaining highlighting nuclei.

DIRAS3 Antibody by FACS in K562 cells. Flow cytometry analysis using GTP-binding protein Di-Ras3 Antibody shows a rightward shift of the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank (red). K562 cells were fixed with 4% paraformaldehyde. Primary antibody was applied at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC, followed by a fluorescent anti-rabbit IgG secondary antibody. The histogram demonstrates detectable antibody-associated fluorescence under these assay conditions.

DIRAS3 Antibody by WB in human cell lines and rodent brain. Western blot analysis using GTP-binding protein Di-Ras3 Antibody detects a prominent band near 30 kDa, compared with the calculated molecular weight of approximately 26 kDa. Lanes 1-3 contain K562, HeLa and A549 whole-cell lysates; lanes 4 and 5 contain rat and mouse brain tissue lysates. Reduced lysates containing 30 ug total protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection employed an HRP-conjugated anti-rabbit secondary antibody and chemiluminescent substrate.