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Home >> Antibodies >> Dipeptidyl peptidase 3 Antibody / DPP3

Dipeptidyl peptidase 3 Antibody / DPP3 (F54584)

  Catalog No Formulation Size Price (USD)  
Image F54584-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54584-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human uterus tissue with Dipeptidyl peptidase 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Dipeptidyl peptidase 3 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HL60 cell lysate with Dipeptidyl peptidase 3 antibody. Expected molecular weight ~83 kDa and ~70 kDa (DPP3-BBS1).
Flow cytometry testing of human HL60 cells with Dipeptidyl peptidase 3 antibody; Blue=isotype control, Green= Dipeptidyl peptidase 3 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9NY33
Localization Cytoplasmic
Applications Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Limitations This Dipeptidyl peptidase 3 antibody is available for research use only.
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Description

This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Dipeptidyl peptidase 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 583-612 from the human protein was used as the immunogen for the Dipeptidyl peptidase 3 antibody.

Storage

Aliquot the Dipeptidyl peptidase 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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