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Home >> Antibodies >> DIO2 Antibody / Type II Iodothyronine Deiodinase

DIO2 Antibody / Type II Iodothyronine Deiodinase (F54462)

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  Catalog No Formulation Size Price (USD)  
Image F54462-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F54462-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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Western blot testing of 1) mouse heart and 2) human skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
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Western blot testing of 1) mouse heart and 2) human skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of human 1) heart and 2) skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
Western blot testing of human MCF7 cell lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.
IHC testing of FFPE mouse brain tissue with DIO2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MCF7 cells with DIO2 antibody; Blue=isotype control, Green= DIO2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt Q92813
Applications Western Blot : 1:500-1:2000
Flow Cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This DIO2 antibody is available for research use only.
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Description

DIO2 belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3).

Application Notes

The stated application concentrations are suggested starting points. Titration of the DIO2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 165-191 from the human protein was used as the immunogen for the DIO2 antibody.

Storage

Aliquot the DIO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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