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Home >> Antibodies >> Dimethylargininase 1 Antibody / DDAH1

Dimethylargininase 1 Antibody / DDAH1 (F54789)

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  Catalog No Formulation Size Price (USD)  
Image F54789-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54789-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE mouse kidney tissue with Dimethylargininase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE mouse kidney tissue with Dimethylargininase 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse kidney tissue lysate with Dimethylargininase 1 antibody. Expected molecular weight: 31-38 kDa.
Immunofluorescent staining of human HepG2 cells with Dimethylargininase 1 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HEK293 cells with Dimethylargininase 1 antibody; Blue=isotype control, Green= Dimethylargininase 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt O94760
Localization Cytoplasmic
Applications Immunofluorescence : 1:25
Western blot : 1:500-1:1000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This Dimethylargininase 1 antibody is available for research use only.
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Description

DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Dimethylargininase 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 221-250 from the human protein was used as the immunogen for the Dimethylargininase 1 antibody.

Storage

Aliquot the Dimethylargininase 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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