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Home >> Antibodies >> Diacylglycerol kinase zeta Antibody / DGKZ

Diacylglycerol kinase zeta Antibody / DGKZ (RQ6125)

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  Catalog No Formulation Size Price (USD)  
Image RQ6125 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human breast cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Diacylglycerol kinase zeta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human T-47D, 2) human ThP-1, 3) rat brain and 4) mouse brain lysate with Diacylglycerol kinase zeta antibody. Predicted molecular weight ~124 kDa.
Flow cytometry testing of human HL60 cells with Diacylglycerol kinase zeta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Diacylglycerol kinase zeta antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q13574
Localization Nuclear, cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Diacylglycerol kinase zeta antibody is available for research use only.
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Description

Diacylglycerol kinase zeta is an enzyme that in humans is encoded by the DGKZ gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.

Application Notes

Optimal dilution of the Diacylglycerol kinase zeta antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids E664-V928) was used as the immunogen for the Diacylglycerol kinase zeta antibody.

Storage

After reconstitution, the Diacylglycerol kinase zeta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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