DHX16 Antibody | FY13629 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	FY13629	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

DHX16 Antibody by IF in U2OS cells. Immunofluorescence analysis using DEAH-box helicase 16 Antibody reveals strong green nuclear staining in U2OS cells. Red alpha-tubulin counterstaining highlights the cytoskeleton. Cells were treated with enzyme-based antigen retrieval and incubated with 5 ug/mL primary antibody overnight at 4oC before fluorescent secondary antibody detection. The image depicts the observed staining pattern without establishing organelle-specific colocalization.

DHX16 Antibody by WB in human cell lines. Western blot analysis using DEAH-box helicase 16 Antibody detects a prominent immunoreactive band consistent with the expected molecular weight of approximately 119 kDa. Lanes 1-3 contain Jurkat, K562 and SiHa whole-cell lysates, respectively. Reduced samples containing 30 ug total protein per lane were resolved by 8% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, incubated with 0.5 ug/mL primary antibody overnight at 4oC, and probed with an HRP-conjugated secondary antibody. Signal was visualized by chemiluminescence.

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DHX16 Antibody by IF in U2OS cells. Immunofluorescence analysis using DEAH-box helicase 16 Antibody reveals strong green nuclear staining in U2OS cells. Red alpha-tubulin counterstaining highlights the cytoskeleton. Cells were treated with enzyme-based antigen retrieval and incubated with 5 ug/mL primary antibody overnight at 4oC before fluorescent secondary antibody detection. The image depicts the observed staining pattern without establishing organelle-specific colocalization.

DHX16 Antibody by WB in human cell lines. Western blot analysis using DEAH-box helicase 16 Antibody detects a prominent immunoreactive band consistent with the expected molecular weight of approximately 119 kDa. Lanes 1-3 contain Jurkat, K562 and SiHa whole-cell lysates, respectively. Reduced samples containing 30 ug total protein per lane were resolved by 8% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, incubated with 0.5 ug/mL primary antibody overnight at 4oC, and probed with an HRP-conjugated secondary antibody. Signal was visualized by chemiluminescence.

Species Reactivity	Human, Mouse
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit Ig
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
UniProt	O60231
Applications	Western Blot : 0.5-1ug/ml Immunofluorescence : 5ug/ml ELISA : 0.1-0.5 ug/ml
Limitations	This DHX16 Antibody / DEAH-box helicase 16 Antibody is provided for laboratory research use only.
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Description

DHX16 Antibody / DEAH-box helicase 16 Antibody targets an ATP-dependent RNA helicase involved in pre-messenger RNA splicing. DHX16 is a member of the DEAH-box family of proteins, whose conserved helicase cores couple nucleotide hydrolysis to changes in RNA-protein interactions. Splicing removes introns from newly transcribed RNA and joins exons to produce mature transcripts. This process requires a spliceosome that repeatedly rearranges its RNA and protein components as it progresses through assembly, catalysis, and disassembly.

DHX16 is closely related to the yeast splicing factor Prp2, an ATPase involved in remodeling the spliceosome before the first catalytic step. Such remodeling is essential because the spliceosome initially assembles in a configuration that is not yet competent to catalyze intron removal. ATP-driven rearrangements help expose or reposition RNA elements and displace proteins that would otherwise obstruct catalysis. DHX16 is therefore associated with a crucial transition between spliceosome assembly and productive RNA processing.

DEAH-box helicases are sometimes described as RNA unwinding enzymes, but their cellular activities often involve remodeling ribonucleoprotein complexes rather than simply separating complementary RNA strands. Their function depends on interactions with other splicing factors and the structural state of the spliceosome. For DHX16, identifying these interactions is important for distinguishing direct remodeling events from secondary consequences of stalled splicing.

Accurate intron removal is necessary to maintain coding sequences and regulate transcript diversity. Failure at a catalytic transition can cause retention of introns, abnormal splice products, or degradation of incompletely processed RNA. Because many genes rely on coordinated splicing, disruption of a central remodeling factor can affect numerous downstream cellular pathways.

NSJ Bioreagents offers this DHX16 Antibody to facilitate investigation of ATP-dependent spliceosome remodeling and the events that permit pre-mRNA splicing to proceed. Understanding DHX16's position in the splicing cycle can help clarify how dynamic RNA-protein assemblies achieve the precision needed for reliable gene expression.

Explore related research targets in our Cell Biology Antibodies collection.

Application Notes

Investigators should determine the optimal dilution of the DHX16 Antibody / DEAH-box helicase 16 Antibody empirically for their experimental conditions.

Immunogen

The DHX16 Antibody was generated using e. coli-derived human DHX16 recombinant protein (amino acids M1-G1041).

Storage

Store the reconstituted DHX16 Antibody at 4oC for up to one month. For long-term storage, divide into aliquots and keep at -20oC for up to six months. Avoid repeated freeze-thaw cycles.

Alternate Names

DHX16 antibody, Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 antibody, DEAH-box helicase 16 antibody, EC:3.6.4.13 antibody, DEAH-box protein 16 antibody, DEAH box protein 16 antibody, DDX16 antibody, DBP2 antibody

DHX16 Antibody / DEAH-box helicase 16 Antibody (FY13629)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents