DHX15 Antibody / Prp43 (RQ5627)

Immunofluorescent staining of FFPE human U-2 OS cells with DHX15 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human prostate cancer tissue with DHX15 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with DHX15 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testicular germ cell tumor tissue with DHX15 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with DHX15 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) human 293T, 3) human Caco-2, 4) human HEL, 5) human RT4, 6) human A549, 7) human A431, 8) human U-251, 9) rat C6 and 10) mouse thymus tissue lysate with DHX15 antibody. Predicted molecular weight ~91 kDa.

Flow cytometry testing of fixed and permeabilized human HeLa cells with DHX15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DHX15 antibody.