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Home >> Antibodies >> DHFR Antibody

DHFR Antibody (R31590)

  Catalog No Formulation Size Price (USD)  
Image R31590 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of DHFR antibody and Lane 1: rat liver; 2: mouse liver; 3: human HepG2; 4: (h) Jurkat. Expected molecular weight 21~24 kDa.
Western blot testing of DHFR antibody and recombinant human protein (0.5ng)
IHC-P: DHFR antibody testing of human lung cancer tissue
IHC-P staining of mouse brain tissue
IHC-P staining of rat brain tissue
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 1719
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This DHFR antibody is available for research use only.
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Description

Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. It is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2-187) was used as the immunogen for this DHFR antibody.

Storage

After reconstitution, the DHFR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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