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Home >> Antibodies >> DHFR Antibody

DHFR Antibody (F50355)

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  Catalog No Formulation Size Price (USD)  
Image F50355-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50355-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.
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Western blot analysis of DHFR antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the DHFR gene. Predicted molecular weight ~22 kDa.
DHFR antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma.
Western blot analysis of DHFR antibody and mouse liver tissue lysate
Availability 1-3 business days
Species Reactivity Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P00375
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Limitations This DHFR antibody is available for research use only.
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Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Application Notes

Titration of the DHFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 135-164 from mouse Dihydrofolate reductase was used as the immunogen for this DHFR antibody.

Storage

Aliquot the DHFR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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