DGAT1 Antibody / Diacylglycerol O-acyltransferase 1 (R35384)

DGAT1 Antibody Rat Duodenum WB. Western blot analysis of DGAT1 expression was performed using anti-DGAT1 antibody in rat duodenum lysate. DGAT1, also known as Diacylglycerol O-Acyltransferase 1, catalyzes the final step of triglyceride synthesis and is highly expressed in intestinal tissues involved in dietary lipid absorption. A specific immunoreactive band is detected at approximately 45-55 kDa, consistent with the predicted molecular weight of DGAT1 (~55 kDa). Prominent expression in the duodenum is in agreement with the established role of DGAT1 in re-esterification of dietary fatty acids and chylomicron assembly. These results support the utility of DGAT1 Antibody for studies of lipid metabolism, intestinal fat absorption, and energy homeostasis.

DGAT1 Antibody Human Colon IHC. IHC staining of FFPE human colon tissue with DGAT1 antibody demonstrated strong cytoplasmic staining in glandular epithelial cells, consistent with the role of DGAT1 (Diacylglycerol O-Acyltransferase 1) in triglyceride synthesis and intestinal lipid metabolism. DGAT1 is highly expressed in enterocytes, where it catalyzes the final step of triglyceride biosynthesis and facilitates dietary fat absorption. The prominent epithelial staining observed in the colon is in agreement with the established involvement of DGAT1 in neutral lipid storage and energy homeostasis. Staining was performed using an alkaline phosphatase detection system. Heat-induced epitope retrieval was achieved by steaming tissue sections in pH 6 citrate buffer before immunostaining with DGAT1 antibody at 3.75 ug/ml.