DGAT1 Antibody / Triglyceride Synthesis Enzyme Antibody [clone 23D11] (RQ8914)

DGAT1 Antibody Human Stomach Cancer IHC. IHC staining of FFPE human stomach cancer tissue with DGAT1 antibody revealed strong cytoplasmic and membranous staining in malignant epithelial cells, consistent with the role of DGAT1 (Diacylglycerol O-Acyltransferase 1) in triglyceride synthesis and lipid droplet formation. DGAT1 is a key regulator of neutral lipid metabolism and has been implicated in metabolic adaptation and tumor cell survival. The widespread staining pattern observed in tumor cells is consistent with increased lipid biosynthesis and energy storage requirements associated with cancer progression. Staining was performed using an HRP-conjugated secondary antibody and DAB chromogen. Heat-induced epitope retrieval was achieved by boiling tissue sections in pH 8 EDTA buffer for 20 minutes followed by cooling before immunostaining.

DGAT1 Antibody Human Cell Lysates WB. Western blot analysis of DGAT1 expression was performed using anti-DGAT1 antibody in lysates from HeLa, PC-3, HepG2, and Caco-2 cells. DGAT1, also known as Diacylglycerol O-Acyltransferase 1, catalyzes the final step of triglyceride synthesis and plays a critical role in lipid storage and energy homeostasis. A specific immunoreactive band is detected at approximately 45-55 kDa in all four cell lines, consistent with the predicted molecular weight of DGAT1 (~55 kDa). Similar expression across cervical, prostate, liver, and intestinal cell lines reflects the widespread requirement for triglyceride biosynthesis and lipid droplet formation in diverse cell types. These results support the utility of DGAT1 Antibody for studies of lipid metabolism, energy homeostasis, and metabolic disease.