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Home >> Antibodies >> DFFB Antibody / DNA fragmentation factor subunit beta

DFFB Antibody / DNA fragmentation factor subunit beta (F55043)

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  Catalog No Formulation Size Price (USD)  
Image F55043-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F55043-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human prostate tissue with DFFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human prostate tissue with DFFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) HepG2, 3) Jukat, 4) LNCaP and 5) Raji cell lysate with DFFB antibody. Predicted molecular weight ~39 kDa.
Flow cytometry testing of human HEK293 cells with DFFB antibody; Blue=isotype control, Green= DFFB antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O76075
Localization Cytoplasmic, nuclear
Applications Western blot : 1:500-1:1000
Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Limitations This DFFB antibody is available for research use only.
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Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the DFFB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the DFFB antibody.

Storage

Aliquot the DFFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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