DSG3 Antibody - Microarray Validated | Desmoglein 3 [clone DSG3/2840] (V7721)

Immunohistochemistry of DSG3 Antibody - Microarray Validated clone DSG3/2840 in human esophageal carcinoma tissue. Formalin-fixed, paraffin-embedded esophageal carcinoma demonstrates strong membranous staining of tumor epithelial cells, consistent with Desmoglein 3 localization at desmosomal cell-cell junctions in stratified squamous epithelium-derived malignancy. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes followed by cooling prior to staining. The microarray validated monoclonal antibody clone DSG3/2840 was used as the primary antibody.

IHC staining of FFPE human esophageal carcinoma with DSG3 antibody-microarray validated clone DSG3/2840. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Human protein microarray specificity validation of DSG3 Antibody - Microarray Validated clone DSG3/2840. Analysis was performed using the HuProt(TM) microarray containing more than 19,000 full-length human proteins. The antibody demonstrates the highest signal intensity for Desmoglein 3, ranking first among all proteins tested with a strong Z-score and substantial separation from the next highest ranked proteins, supporting high target specificity of clone DSG3/2840.
Z- and S-score explanation: The Z-score represents the strength of signal generated when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed in units of standard deviations above the mean signal of all proteins on the array. Proteins are ranked in descending order according to Z-score. The S-score represents the difference in Z-scores between adjacent ranked proteins and reflects the relative specificity of the antibody for its intended target.

SDS-PAGE analysis of purified, BSA-free DSG3 antibody-microarray validated clone DSG3/2840 as confirmation of integrity and purity.