Desmoglein 3 Antibody / Squamous Epithelial Marker / DSG3 [clone DSG3/2838] (V7932)

Desmoglein 3 Antibody Esophageal Carcinoma IHC. Immunohistochemistry staining of formalin-fixed, paraffin-embedded human esophageal carcinoma tissue using HuProt validated monoclonal clone DSG3/2838 demonstrates strong membranous HRP-DAB brown staining in malignant squamous epithelial cells, consistent with localization of Desmoglein 3 / DSG3 at desmosomal cell-cell junctions within stratified squamous epithelium-derived tumor tissue. The observed staining pattern supports the established role of DSG3 as a squamous epithelial differentiation and desmosomal adhesion marker. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling prior to staining.

Desmoglein 3 Antibody Squamous Cell Junction IHC. Immunohistochemistry staining of FFPE human esophageal carcinoma tissue using HuProt validated monoclonal clone DSG3/2838 demonstrates strong membranous HRP-DAB brown staining outlining malignant squamous epithelial cells, consistent with localization of Desmoglein 3 / DSG3 at desmosomal junctions within stratified epithelial-derived carcinoma tissue. The staining prominently highlights epithelial cell-cell adhesion architecture characteristic of squamous differentiation-associated tumors. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Desmoglein 3 Antibody Protein Microarray Specificity Analysis. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HuProt validated monoclonal clone DSG3/2838 identified Desmoglein 3 as the dominant target with a Z-score of 131.23 and an S-score of 96.2, supporting exceptionally strong target specificity relative to unrelated proteins present on the array. The markedly elevated S-score demonstrates highly preferential recognition of DSG3 with minimal off-target reactivity. The Z-score represents signal intensity generated by antibody binding relative to the mean signal across the array, while the S-score reflects the difference in signal strength between successive ranked proteins and serves as a measure of relative target specificity.

SDS-PAGE analysis of purified, BSA-free Desmoglein 3 antibody (clone DSG3/2838) as confirmation of integrity and purity.