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Home >> Antibodies >> Desmoglein 2 Antibody / DSG2

Desmoglein 2 Antibody / DSG2 (RQ5646)

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  Catalog No Formulation Size Price (USD)  
Image RQ5646 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human prostate cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human appendicitis tissue with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Desmoglein 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human MCF7 cells with Desmoglein 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) K562, 3) HeLa, 4) U-2 OS, 5) HepG2 and 6) PC-3 lysate with Desmoglein 2 antibody. Expected molecular weight: 122-160 kDa depending on glycosylation level.
Flow cytometry testing of human U-87 MG cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
Flow cytometry testing of rat RH35 cells with Desmoglein 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmoglein 2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q14126
Localization Cytoplasm, membrane (cell junctions)
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Desmoglein 2 antibody is available for research use only.
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Description

Desmoglein-2 is a protein that in humans is encoded by the DSG2 gene. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in DSG2 display a high degree of penetrance. Disease expression was of variable severity with LV involvement a prominent feature. The low prevalence of classical ECG changes highlights the need to expand current diagnostic criteria to take account of LV disease, childhood disease expression, and incomplete penetrance.

Application Notes

Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids L24-E1020) was used as the immunogen for the Desmoglein 2 antibody.

Storage

After reconstitution, the Desmoglein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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