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Home >> Antibodies >> Desmin Antibody

Desmin Antibody [clone 2B5] (RQ5525)

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  Catalog No Formulation Size Price (USD)  
Image RQ5525 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human skeletal muscle with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human skeletal muscle with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human esophagus squama cancer with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle, 5) human K562 and 6) rat liver lysate with Desmin antibody. Predicted molecular weight ~54 kDa.
Flow cytometry testing of human ThP-1 cells with Desmin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Desmin antibody.
IHC staining of FFPE mouse skeletal muscle with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat skeletal muscle with Desmin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 2B5
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P17661
Localization Cytoplasmic, membranous
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Desmin antibody is available for research use only.
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Description

DES, also called desmin, is a protein that in humans is encoded by the DES gene, and this gene is mapped to 2q35. DES is the muscle-specific member of the intermediate filament (IF) protein family. It is one of the earliest myogenic markers, both in heart and somites, and is expressed in satellite stem cells and replicating myoblasts. DES is very important in muscle cell architecture and structure since it connects many components of the cytoplasm. It may be also play an important role in mitochondria function. What�s more, DES provides attachments between the terminal Z disc and membrane-associated proteins to form a force-transmitting system that parallels the thin filaments at myotendinous junctions.

Application Notes

Optimal dilution of the Desmin antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids M1-T304) was used as the immunogen for the Desmin antibody.

Storage

After reconstitution, the Desmin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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