- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
DEPTOR Antibody / DEPDC6 (FY12970)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12970 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
DEPTOR antibody detects DEP domain-containing mTOR-interacting protein, an endogenous inhibitor of the mTORC1 and mTORC2 complexes. The UniProt recommended name is DEP domain-containing mTOR-interacting protein (DEPTOR), also known as DEP domain-containing protein 6 (DEPDC6). DEPTOR acts as a critical regulator of cell growth, metabolism, and survival by directly binding to mechanistic target of rapamycin (mTOR), suppressing its kinase activity.
Functionally, DEPTOR antibody recognizes a 409-amino-acid cytoplasmic protein that negatively regulates mTOR signaling. DEPTOR binds to both mTORC1 and mTORC2 via its PDZ domain, preventing phosphorylation of downstream effectors such as S6K1 and AKT. Its expression is finely tuned by nutrient availability, growth factors, and stress stimuli. Under normal conditions, mTOR phosphorylates DEPTOR, marking it for ubiquitination and proteasomal degradation, thereby sustaining mTOR activity. Conversely, nutrient deprivation or stress increases DEPTOR levels, suppressing mTOR signaling to promote autophagy and energy conservation.
The DEPTOR gene is located on chromosome 8q24.12 and encodes a DEP and PDZ domain-containing protein that integrates environmental cues with cellular metabolic state. DEPTOR functions as a feedback regulator ensuring mTOR pathway balance. Dysregulation of DEPTOR disrupts this feedback loop, leading to aberrant cell growth and metabolic reprogramming. In cancer, overexpression of DEPTOR has been observed in multiple myeloma, where it paradoxically supports survival by maintaining AKT activation through mTORC2 inhibition.
DEPTOR antibody is used to explore cellular signaling pathways involving mTOR, autophagy, and nutrient sensing. It supports applications such as western blotting, immunoprecipitation, and fluorescence microscopy to monitor DEPTOR expression and mTOR complex association. In metabolic studies, DEPTOR serves as a marker for energy stress adaptation, linking insulin signaling and amino acid availability to cell growth control. Elevated DEPTOR expression modulates lipid biosynthesis and glucose uptake pathways, reinforcing its role in metabolic regulation.
Structurally, DEPTOR contains an N-terminal DEP domain responsible for membrane association and a C-terminal PDZ domain mediating protein-protein interactions with mTOR and associated complexes. Post-translational modifications including phosphorylation and ubiquitination govern its stability and turnover. DEPTOR also interacts with transcription factors and kinases outside the mTOR axis, expanding its regulatory network. NSJ Bioreagents provides DEPTOR antibody reagents validated for use in signaling, oncology, and metabolism research to investigate mTOR regulation and stress adaptation.
Optimal dilution of the DEPTOR antibody should be determined by the researcher.
E.coli-derived human DEPDC6/DEPTOR recombinant protein (Position: A22-C409) was used as the immunogen for the DEPTOR antibody.
After reconstitution, the DEPTOR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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