deltaNp63 Antibody Clone ZR8 / p40 Monoclonal Antibody [clone ZR8] (V8629)

deltaNp63 Antibody Clone ZR8. Immunohistochemistry analysis of Tumor protein p40 (TP63) in FFPE human skin using deltaNp63 Antibody Clone ZR8 demonstrates strong HRP-DAB brown nuclear staining in basal keratinocytes along the epidermal layer, with suprabasal cells showing reduced or absent staining. The nuclear-restricted pattern highlights the basal cell compartment and aligns with the known distribution of deltaNp63 in stratified squamous epithelium. Staining is crisp with minimal background, allowing clear delineation of epithelial layering and cellular organization. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human skin with deltaNp63 antibody (clone ZR8). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

deltaNp63 Antibody Clone ZR8. Immunofluorescence analysis of PFA-fixed and permeabilized human HeLa cells using deltaNp63 Antibody Clone ZR8 demonstrates strong green nuclear staining corresponding to Tumor protein p40 (TP63), while Phalloidin (red) highlights actin cytoskeletal structures in the cytoplasm. The fluorescence signal is confined to nuclei, consistent with the role of deltaNp63 as a nuclear transcription factor, with minimal cytoplasmic background. The clear nuclear localization and strong signal intensity support specific detection of deltaNp63-positive cells. The distinct separation between nuclear p40 signal and cytoskeletal staining enables accurate interpretation of subcellular localization and cellular organization in immunofluorescence-based imaging.

deltaNp63 Antibody Clone ZR8. Flow cytometry analysis of PFA-fixed and permeabilized human HeLa cells using deltaNp63 Antibody Clone ZR8 demonstrates a clear rightward shift in fluorescence intensity for the p40 (TP63)-stained population (blue) compared to the isotype control (red). This separation indicates specific intracellular detection of deltaNp63 with minimal background signal. The distinct population shift supports accurate gating and quantification of deltaNp63-positive cells, consistent with nuclear expression of this transcription factor following permeabilization. The rabbit monoclonal format of clone ZR8 provides strong signal resolution and reproducible performance in flow cytometry-based detection of epithelial lineage-associated markers.