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Home >> Antibodies >> Decorin Antibody

Decorin Antibody [clone DCN/6289] (V4008)

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  Catalog No Formulation Size Price (USD)  
Image V4008-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4008-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4008SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human placental tissue with Decorin antibody (clone DCN/6289) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.
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IHC staining of FFPE human placental tissue with Decorin antibody (clone DCN/6289) at 2ug/ml. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.
IHC staining of FFPE human prostate tissue with Decorin antibody (clone DCN/6289). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, lambda
Clone Name DCN/6289
Purity Protein A/G affinity
UniProt P07585
Localization Secreted
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Decorin antibody is available for research use only.
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Description

Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGF may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Application Notes

Optimal dilution of the Decorin antibody should be determined by the researcher.

Immunogen

Recombinant full-length protein was used as the immunogen for the Decorin antibody.

Storage

Aliquot the Decorin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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