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Home >> Antibodies >> Death-associated protein kinase 3 Antibody / Dapk3 / Zip kinase

Death-associated protein kinase 3 Antibody / Dapk3 / Zip kinase (RQ7973)

  Catalog No Formulation Size Price (USD)  
Image RQ7973 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human RM-1 cells with Death-associated protein kinase 3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) mouse lung, 2) mouse stomach, 3) rat kidney and 4) rat PC-12 cell lysate with Death-associated protein kinase 3 antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of mouse HEPA1-6 cells with Death-associated protein kinase 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Death-associated protein kinase 3 antibody.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O54784
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Death-associated protein kinase 3 antibody is available for research use only.
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Description

Death-associated protein kinase 3 is an enzyme that in humans is encoded by the DAPK3 gene. Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.

Application Notes

Optimal dilution of the Death-associated protein kinase 3 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant mouse protein (amino acids R290-R448) was used as the immunogen for the Death-associated protein kinase 3 antibody.

Storage

After reconstitution, the Death-associated protein kinase 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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