Death-associated protein kinase 2 Antibody / DAPK2 (F54911)

Death-associated Protein Kinase 2 Antibody Human Skeletal Muscle IHC. Immunohistochemistry staining of FFPE human skeletal muscle tissue using Death-associated protein kinase 2 antibody demonstrates diffuse cytoplasmic staining throughout skeletal myofibers, consistent with expression of Death Associated Protein Kinase 2 (DAPK2). DAPK2 is a calcium/calmodulin-regulated serine/threonine kinase that participates in signaling pathways involved in cellular stress responses, apoptosis regulation, autophagy, and maintenance of tissue homeostasis. The observed staining pattern is consistent with the intracellular localization expected for a signaling kinase and supports the utility of this antibody for studies of kinase-mediated regulatory pathways in muscle biology and cellular adaptation. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Death-associated Protein Kinase 2 Antibody Mouse Lung WB. Western blot analysis of mouse lung tissue lysate using Death-associated protein kinase 2 antibody demonstrates detection of a distinct immunoreactive band at approximately 43 kDa, consistent with the predicted molecular weight of Death Associated Protein Kinase 2 (DAPK2). DAPK2 is a calcium/calmodulin-regulated serine/threonine kinase that functions in signaling pathways involved in apoptosis regulation, cellular stress responses, autophagy, and maintenance of tissue homeostasis. The observed band supports expression of DAPK2 in lung tissue and demonstrates the utility of this antibody for studies of kinase-mediated signaling, programmed cell death pathways, and cellular regulatory mechanisms. Predicted molecular weight: ~43 kDa.

Death-associated Protein Kinase 2 Antibody Transfected Cell WB. Western blot analysis of non-transfected and DAPK2-transfected human 293 cell lysates using Death-associated protein kinase 2 antibody demonstrates a strong immunoreactive band at approximately 43 kDa in the transfected sample, with little to no corresponding signal detected in the non-transfected control. The detected band is consistent with the expected molecular weight of Death Associated Protein Kinase 2 (DAPK2), a calcium/calmodulin-regulated serine/threonine kinase involved in apoptosis regulation, cellular stress responses, autophagy, and intracellular signaling pathways. The selective detection of DAPK2 in transfected cells supports the specificity of the antibody for its intended target. Expected molecular weight: ~43 kDa.