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Home >> Antibodies >> Death-associated protein kinase 2 Antibody / DAPK2

Death-associated protein kinase 2 Antibody / DAPK2 (F54911)

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  Catalog No Formulation Size Price (USD)  
Image F54911-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F54911-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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IHC testing of FFPE human skeletal muscle tissue with Death-associated protein kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human skeletal muscle tissue with Death-associated protein kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse lung tissue lysate with Death-associated protein kinase 2 antibody. Predicted molecular weight ~43 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Death-associated protein kinase 2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q9UIK4
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:50-1:100
Western Blot : 1:500-1:1000
Limitations This Death-associated protein kinase 2 antibody is available for research use only.
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Description

DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Death-associated protein kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 40-70 from the human protein was used as the immunogen for the Death-associated protein kinase 2 antibody.

Storage

Aliquot the Death-associated protein kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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