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Home >> Antibodies >> DDT Antibody

DDT Antibody (R30113)

  Catalog No Formulation Size Price (USD)  
Image R30113 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human placental tissue with DDT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human HCCT, 2) rat liver and 3) mouse liver tissue lysate with DDT antibody. Predicted molecular weight ~13 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 0.025% sodium azide
UniProt P30046
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested)
Limitations This DDT antibody is available for research use only.
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Description

DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the DDT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human DDT recombinant protein (AA 1-118) was used as the immunogen for this DDT antibody.

Storage

After reconstitution, the DDT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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