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Home >> Antibodies >> DCTN5 Antibody / Dynactin subunit 5 Antibody

DCTN5 Antibody / Dynactin subunit 5 Antibody (FY13656)

  Catalog No Formulation Size Price (USD)  
Image FY13656 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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DCTN5 Antibody by IHC in Human Pancreatic Cancer Tissue. Immunohistochemistry analysis of DCTN5 expression in a paraffin-embedded section of human pancreatic cancer tissue using DCTN5 Antibody. Brown DAB staining is prominent in the cytoplasm of malignant epithelial cells, with variable intensity across the tumor. Hematoxylin counterstaining highlights the nuclei, while adjacent connective tissue shows comparatively weaker immunoreactivity. The staining pattern demonstrates DCTN5 detection in the examined pancreatic tumor section.
DCTN5 Antibody by IHC in Human Liver Cancer Tissue. Immunohistochemistry analysis of DCTN5 expression in a paraffin-embedded section of human liver cancer tissue using DCTN5 Antibody. Strong brown DAB staining is visible throughout numerous tumor cells, predominantly in the cytoplasm. The staining is heterogeneous, with variable intensity among individual cells. Nuclei are counterstained blue with hematoxylin, and the surrounding stromal regions exhibit comparatively limited immunoreactivity.
DCTN5 Antibody by WB in six human cell lines. Western blot analysis of MCF-7, SH-SY5Y, HepG2, K562, PC-12 and Neuro-2a lysates, shown in lanes 1-6, respectively, using DCTN5 Antibody. Immunoreactive bands are visible predominantly near 15-16 kDa, with a faint signal near 20 kDa in selected samples. The observed major bands are consistent with the expected low molecular weight of dynactin subunit 5. Signal intensity varies among the lysates. The blot includes a molecular weight marker at left; no identity is assigned to additional weak signals without independent validation.
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Buffer Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
UniProt Q9BTE1
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
ELISA : 0.1-0.5 ug/ml
Limitations This DCTN5 Antibody / Dynactin subunit 5 Antibody is supplied exclusively for research use.
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Description

DCTN5 Antibody / Dynactin subunit 5 Antibody targets a component of dynactin, the multiprotein assembly that enables cytoplasmic dynein to transport cargo along microtubules. DCTN5 encodes the small dynactin subunit commonly called p25. Dynactin links the force-generating motor dynein to cargo adaptors and helps coordinate long-distance movement of vesicles, organelles, and protein complexes. This transport is especially consequential in polarized cells, where materials must move between distant regions of the cytoplasm.

Dynactin contains an actin-related filament built around ARP1, a shoulder complex, and a projecting structure that includes the large p150 subunit. DCTN5 contributes to the pointed-end complex of the ARP1 filament alongside proteins such as DCTN4 and DCTN6. The pointed-end complex helps define dynactin architecture and participates in interactions relevant to cargo engagement. Structural studies of dynein-dynactin assemblies have shown that dynactin is not merely an attachment platform: it organizes dynein motors into productive transport complexes when appropriate activating adaptors are present. Consequently, the contribution of individual dynactin subunits must be considered within the larger assembly rather than as isolated motor proteins.

Dynein-dynactin transport supports endosome positioning, Golgi organization, mitotic spindle function, and redistribution of intracellular components. Defects in this system can be particularly disruptive to neurons because axonal transport must operate across exceptional distances. The precise contribution of p25 to distinct cargo classes remains an active structural and cell-biological question. NSJ Bioreagents offers this DCTN5 Antibody to support investigation of dynactin subunit organization and the mechanisms that connect microtubule motors to intracellular cargo. Defining the interactions of the pointed-end complex may clarify how dynactin achieves cargo selectivity while preserving efficient directional transport.

Explore related research targets in our Cell Biology Antibodies collection.

Application Notes

The optimal working dilution of the DCTN5 Antibody / Dynactin subunit 5 Antibody should be determined empirically by the investigator.

Immunogen

The DCTN5 Antibody was generated using e. coli-derived human DCTN5 recombinant protein (amino acids M1-N113).

Storage

Once reconstituted, refrigerate the DCTN5 Antibody at 4oC for no longer than one month. For extended storage, freeze aliquots at -20oC for up to six months and avoid repeated freeze-thaw cycles.

Alternate Names

DCTN5 antibody, Dynactin subunit 5 antibody, dynactin 5 (p25) antibody, Dynactin subunit p25 antibody, p25 antibody, DCTN5 rabbit polyclonal antibody

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