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Home >> Antibodies >> DCN Antibody for IHC / Decorin

DCN Antibody for IHC / Decorin [clone MSVA-537R] (V6066)

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  Catalog No Formulation Size Price (USD)  
Image V6066-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6066-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry of DCN Antibody for IHC in human tissue microarrays. Recombinant monoclonal clone MSVA-537R was applied to formalin-fixed, paraffin-embedded normal and cancer tissues. In normal tissues, Decorin expression is predominantly observed within stromal extracellular matrix compartments, highlighting collagen-rich connective tissue surrounding glands, vessels, and muscle bundles, while epithelial and parenchymal cells show minimal staining. In cancer tissues, variable stromal staining intensity is present within tumor-associated connective tissue, consistent with extracellular matrix remodeling patterns. The observed staining distribution aligns with reported DCN expression profiles in the Human Protein Atlas.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-537R
UniProt P07585
Localization Extracellular matrix, Extracellular space, Secreted
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This DCN/Decorin antibody is available for research use only.
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Description

DCN Antibody for IHC detects Decorin, a small leucine-rich extracellular matrix proteoglycan encoded by the DCN gene and widely distributed in connective tissues. Clone MSVA-537R is a recombinant monoclonal antibody developed for immunohistochemistry and supports visualization of Decorin within stromal extracellular matrix compartments of formalin-fixed tissue sections.

DCN antibody, also referred to as Decorin antibody and small leucine-rich proteoglycan decorin antibody in the literature, recognizes a secreted proteoglycan characterized by tandem leucine-rich repeat domains and a single dermatan sulfate or chondroitin sulfate glycosaminoglycan chain. Decorin binds to fibrillar collagens, particularly type I collagen, where it regulates collagen fibrillogenesis, fiber diameter, and interfibrillar spacing. Through these interactions, Decorin contributes to tissue tensile strength and extracellular matrix stability.

Beyond structural support, Decorin modulates cellular signaling pathways through interactions with growth factors and receptor tyrosine kinases, including transforming growth factor beta and epidermal growth factor receptor. These regulatory effects influence cell proliferation, differentiation, and migration, positioning Decorin as both a matrix scaffold and a signaling modulator within the tissue microenvironment. DCN expression is prominent in skin, tendon, ligament, placenta, prostate stroma, and other collagen-rich tissues. In immunohistochemical analyses, Decorin typically demonstrates extracellular stromal staining between collagen bundles and surrounding glandular or vascular structures.

Altered Decorin expression has been associated with fibrosis, abnormal wound healing, and tumor progression, where remodeling of collagen architecture and stromal signaling contributes to disease development. Assessment of Decorin distribution may therefore support research into extracellular matrix biology, desmoplastic reactions, and connective tissue organization. This DCN Antibody for IHC targets Decorin in research applications and is available from NSJ Bioreagents.

Application Notes

1. Optimal dilution of the DCN/Decorin antibody should be determined by the researcher.

2. This DCN antibody for IHC is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

4. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Immunogen

Recombinant human Decorin protein fragment (around amino acids 212-336) (exact sequence is proprietary) was used as the immunogen for the DCN antibody for IHC.

Storage

DCN/Decorin antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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