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Home >> Antibodies >> DCK Antibody

DCK Antibody [clone 3G10] (RQ5558)

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  Catalog No Formulation Size Price (USD)  
Image RQ5558 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human tonsil with DCK antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with DCK antibody. HIER: boil tissue sections in pH8 EDTA buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of human U-2 OS cells with DCK antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEK293, 2) HeLa, 3) HepG2, 4) Jurkat and 5) Raji cell lysate with DCK antibody. Predicted molecular weight ~30 kDa.
Flow cytometry testing of human 293T cells with DCK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 3G10
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P27707
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Limitations This DCK antibody is available for research use only.
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Description

Deoxycytidine kinase (dCK) is an enzyme which is encoded by the DCK gene in humans. Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Application Notes

Optimal dilution of the DCK antibody should be determined by the researcher.

Immunogen

A human recombinant protein (amino acids E17-L260) was used as the immunogen for the DCK antibody.

Storage

After reconstitution, the DCK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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