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Home >> Antibodies >> DCK Antibody / Deoxycytidine kinase

DCK Antibody / Deoxycytidine kinase (RQ4200)

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  Catalog No Formulation Size Price (USD)  
Image RQ4200 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) mouse spleen, 2) mouse thymus and human 3) HeLa, 4) U-87 MG, 5) MCF7 and 6) U-2 OS lysate with DCK antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
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Western blot testing of 1) mouse spleen, 2) mouse thymus and human 3) HeLa, 4) U-87 MG, 5) MCF7 and 6) U-2 OS lysate with DCK antibody at 0.5ug/ml. Predicted molecular weight ~30 kDa.
IHC staining of FFPE human lung cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human intestinal cancer with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat small intestine with DCK antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human U-2 OS cells with DCK antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCK antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P27707
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This DCK antibody is available for research use only.
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Description

Deoxycytidine kinase (dCK) is an enzyme which is encoded by the DCK gene in humans. Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity.

Application Notes

Optimal dilution of the DCK antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids E17-L260 was used as the immunogen for the DCK antibody.

Storage

After reconstitution, the DCK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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