DCI Antibody / Dodecenoyl-CoA Delta Isomerase / ECI1 (R30856)

IHC analysis of DCI Antibody / Dodecenoyl-CoA delta isomerase (ECI1). ECI1 expression was examined in a paraffin-embedded section of human liver cancer tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-ECI1 antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Strong cytoplasmic staining is observed in tumor cells, consistent with the expected mitochondrial localization of ECI1 in metabolically active hepatic tissue.

IHC analysis of DCI Antibody / Dodecenoyl-CoA delta isomerase (ECI1). ECI1 expression was examined in a paraffin-embedded section of human colon cancer tissue. Following heat-mediated antigen retrieval in EDTA buffer (pH 8.0), tissue sections were blocked with goat serum and incubated with a rabbit anti-ECI1 antibody. Immunoreactivity was visualized using an HRP-based detection system with DAB chromogen. Cytoplasmic staining is observed in tumor epithelial cells, consistent with the mitochondrial localization of ECI1, while surrounding stromal areas show lower background signal.

Western blot analysis of DCI Antibody / Dodecenoyl-CoA delta isomerase (ECI1). Proteins were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane for immunodetection. Lane 1: human COLO320 whole cell lysates; Lane 2: human 293T whole cell lysates; Lane 3: human HeLa whole cell lysates; Lane 4: human MCF-7 whole cell lysates; Lane 5: rat liver tissue lysates; Lane 6: mouse liver tissue lysates. A single predominant band corresponding to ECI1 is detected at approximately 28 kDa across multiple samples. Although the predicted molecular weight of ECI1 based on amino acid sequence is approximately 33 kDa, mitochondrial matrix enzymes such as ECI1 are well documented to migrate at a lower apparent molecular weight on SDS-PAGE. This behavior is commonly attributed to compact protein folding, limited glycosylation, and sequence-specific electrophoretic properties rather than truncation. The observed banding pattern is consistent with published reports of endogenous ECI1 detected in metabolically active tissues.

Flow cytometry analysis of fixed and permeabilized human 293T cells with DCI antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DCI antibody.