DCAF7 Antibody / DDB1- and CUL4-associated factor 7 Antibody (FY13538)

DCAF7 Antibody by IF in U2OS cells. Immunofluorescence analysis using DDB1- and CUL4-associated factor 7 Antibody reveals green signal prominently associated with nuclei in human U2OS cells, alongside red alpha-tubulin staining of the cytoskeletal network. The merged image distinguishes nuclear DCAF7-associated fluorescence from the filamentous cytoplasmic tubulin signal. Cells underwent enzyme-mediated antigen retrieval for 15 minutes and blocking with 10% goat serum. Both primary antibodies were incubated overnight at 4oC; DyLight 488- and Cy3-conjugated secondary antibodies were applied at 1:500 for 30 minutes at 37oC.

DCAF7 Antibody by WB in human and rodent samples. Western blot analysis using DDB1- and CUL4-associated factor 7 Antibody detects a principal band near 39 kDa in SH-SY5Y (lane 1), MCF-7 (lane 2), HEL (lane 3), K562 (lane 4), rat brain (lane 5), and mouse brain (lane 6), consistent with the expected molecular weight of DCAF7. Reduced lysates containing 30 ug protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% non-fat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Detection used an HRP-linked secondary antibody and chemiluminescent substrate.