DCAF6 Antibody / DDB1- and CUL4-associated factor 6 Antibody (FY13541)

DCAF6 Antibody by IF in HeLa cells. Immunofluorescence analysis using DDB1- and CUL4-associated factor 6 Antibody shows green DCAF6-associated staining in HeLa cells, with red alpha-tubulin staining outlining the cytoskeletal network. The merged image displays green signal over nuclei and adjacent cellular regions against red filamentous staining. Cells underwent enzyme-based antigen retrieval and blocking with 10% goat serum before overnight incubation at 4oC with both primary antibodies at 5 ug/mL. Fluorescent anti-rabbit and anti-mouse secondary antibodies were applied at 1:500, allowing the DCAF6 and alpha-tubulin signals to be visualized together.

DCAF6 Antibody by WB in human and mouse samples. Western blot analysis using DDB1- and CUL4-associated factor 6 Antibody detects a principal band at approximately 96 kDa, matching the calculated DCAF6 mass of 96.3 kDa. Lanes 1 and 2 contain human HEL and K562 whole-cell lysates, respectively; lanes 3 and 4 contain mouse testis and skeletal muscle lysates. Each lane was loaded with 30 ug of reduced protein and separated by 10% SDS-PAGE. The antibody was applied at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection. The observed migration supports detection of DCAF6 in human and mouse specimens.

DCAF6 Antibody by FACS in human HEL cells. Flow cytometry analysis using DDB1- and CUL4-associated factor 6 Antibody shows a right-shifted blue fluorescence histogram relative to the green isotype-control distribution and red unstained control. HEL cells were fixed, permeabilized, and blocked before incubation with 1 ug primary antibody per 1 x 10^6 cells for 30 minutes at 20oC. A fluorescent anti-rabbit secondary antibody was then applied for 30 minutes. The separation of the antibody-stained population from the control distributions demonstrates intracellular DCAF6-associated immunoreactivity under these assay conditions.