DBN1 Monoclonal Antibody ABDC-4 | Drebrin 1

	Catalog No	Formulation	Size	Price (USD)
	FY13676	Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol	100 ul	449
Formulation

DBN1 Monoclonal Antibody WB in Human Cell Lines. Western blot analysis of human HeLa (lane 1), K562 (lane 2), and SH-SY5Y (lane 3) whole cell lysates using the Drebrin 1 Monoclonal Antibody at 1:500. Samples containing 30 ug of total protein per lane were separated by 5-20% SDS-PAGE under reducing conditions. A prominent band is detected at approximately 110-120 kDa in all three samples. Although the calculated molecular weight of DBN1 is approximately 71 kDa, published studies have documented drebrin migration near 120 kDa due to its unusually slow electrophoretic mobility, associated with the protein's acidic character and high proline content.

DBN1 Monoclonal Antibody IHC in Rat Brain. Immunohistochemical staining of FFPE rat brain tissue using the Drebrin 1 Monoclonal Antibody at 1:50. Brown DAB staining is distributed throughout the neuronal processes and surrounding neuropil, with prominent labeling of the cytoplasmic regions and comparatively limited nuclear staining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4Â°C and HRP-DAB detection.

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DBN1 Monoclonal Antibody WB in Human Cell Lines. Western blot analysis of human HeLa (lane 1), K562 (lane 2), and SH-SY5Y (lane 3) whole cell lysates using the Drebrin 1 Monoclonal Antibody at 1:500. Samples containing 30 ug of total protein per lane were separated by 5-20% SDS-PAGE under reducing conditions. A prominent band is detected at approximately 110-120 kDa in all three samples. Although the calculated molecular weight of DBN1 is approximately 71 kDa, published studies have documented drebrin migration near 120 kDa due to its unusually slow electrophoretic mobility, associated with the protein's acidic character and high proline content.

DBN1 Monoclonal Antibody IHC in Rat Brain. Immunohistochemical staining of FFPE rat brain tissue using the Drebrin 1 Monoclonal Antibody at 1:50. Brown DAB staining is distributed throughout the neuronal processes and surrounding neuropil, with prominent labeling of the cytoplasmic regions and comparatively limited nuclear staining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8.0), followed by overnight primary antibody incubation at 4°C and HRP-DAB detection.

Species Reactivity	Human, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG
Clone Name	ABDC-4
Purity	Antigen affinity purified
UniProt	Q16643
Localization	Cytoplasmic
Applications	Western Blot : 1:50 Immunohistochemistry (FFPE) : 1:500
Limitations	This DBN1 Monoclonal Antibody ABDC-4 / Drebrin 1 Monoclonal Antibody is available for research use only.
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Description

DBN1 Monoclonal Antibody ABDC-4 / Drebrin 1 Monoclonal Antibody recognizes drebrin, an actin-binding protein encoded by the DBN1 gene that participates in cytoskeletal organization and neuronal development. Drebrin is particularly abundant in the nervous system, where it contributes to the structural organization of dendritic spines and the regulation of synaptic connections. Through interactions with filamentous actin (F-actin), drebrin influences cytoskeletal dynamics, cell morphology, and the formation of specialized cellular structures.

Drebrin binds along actin filaments and alters their interactions with other actin-associated proteins. Its association with F-actin can influence filament bundling, stability, and remodeling, helping regulate the organization of actin-rich cellular compartments. In neurons, drebrin is enriched in dendritic spines, small protrusions that receive most excitatory synaptic inputs. The actin cytoskeleton within these structures undergoes continuous remodeling during synapse formation and activity-dependent changes in synaptic strength. Drebrin contributes to this process by supporting spine maturation and maintaining the structural organization required for synaptic signaling.

Alternative splicing produces major drebrin isoforms commonly designated drebrin E and drebrin A. Drebrin E is expressed more broadly and is associated with developing neurons and various non-neuronal cells, while drebrin A becomes prominent during neuronal maturation. This developmental transition accompanies changes in dendritic spine morphology and synaptic organization. Drebrin also participates in cell migration, epithelial junction organization, and other processes involving actin remodeling outside the nervous system. Its interactions with additional cytoskeletal regulators help coordinate changes in cell shape and local actin architecture.

Altered drebrin expression and localization have been investigated in neurodegenerative disorders, particularly Alzheimer's disease, where reduced drebrin abundance in vulnerable brain regions has been associated with synaptic deterioration. Experimental studies have also linked drebrin dysregulation to abnormalities in dendritic spine formation, neuronal connectivity, and cognitive function. These findings have made DBN1 an important target in research on synaptic maintenance and neurodegeneration. NSJ Bioreagents offers this DBN1 Monoclonal Antibody for investigating actin cytoskeletal organization, dendritic spine biology, and mechanisms underlying synaptic structural plasticity.

This antibody can be compared with our Drebrin Antibody / DBN1 Neuronal Actin-Binding Protein Antibody (clone DBN1/2880) for consistent detection of DBN1 across neuronal structure and cytoskeletal studies.

Application Notes

Optimal dilution of the DBN1 Monoclonal Antibody ABDC-4 / Drebrin 1 Monoclonal Antibody should be determined by the researcher.

Immunogen

A synthesized peptide derived from human Drebrin was used as the immunogen for the DBN1 Monoclonal Antibody ABDC-4.

Storage

The DBN1 Monoclonal Antibody ABDC-4 can be stored at -20oC.

Alternate Names

Drebrin synaptic antibody, DBN1 spine marker antibody, Drebrin dendritic spine antibody, DBN1 neuronal plasticity antibody, Developmentally regulated brain protein antibody

DBN1 Monoclonal Antibody ABDC-4 / Drebrin 1 Monoclonal Antibody [clone ABDC-4] (FY13676)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents