DBC2 Antibody / RHOBTB2 [clone DBC2/3362] (V8551)

Immunohistochemistry analysis of DBC2 / RHOBTB2 in human thyroid tissue. Formalin-fixed, paraffin-embedded human thyroid tissue was stained with DBC2 antibody. Brown chromogenic signal indicates DBC2-positive epithelial cells within thyroid follicles. Heat-induced epitope retrieval was performed by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human thyroid with DBC2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using DBC2 antibody. These results demonstrate the foremost specificity of the DBC2/3362 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free DBC2 antibody as confirmation of integrity and purity.