DAZL Antibody / Germ Cell Marker Antibody [clone DAZL/4253] (V8666)

DAZL Antibody Testis IHC. Immunohistochemistry staining of FFPE human testis tissue using DAZL Antibody / Germ Cell Marker Antibody (clone DAZL/4253) demonstrates strong cytoplasmic HRP-DAB brown staining within germ cell populations of seminiferous tubules. The staining pattern is consistent with the established expression profile of DAZL, a conserved RNA-binding protein that regulates germline specification, germ cell maintenance, and reproductive development through post-transcriptional control of mRNA translation. DAZL is widely recognized as a marker of germ cell lineage commitment and differentiation, and its expression within seminiferous epithelium is consistent with its essential role in spermatogenesis and male fertility. HIER: boil tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

DAZL Antibody Testis WB. Western blot analysis of human testis lysate using DAZL Antibody / Germ Cell Marker Antibody (clone DAZL/4253) demonstrates a prominent immunoreactive band at approximately 30-35 kDa, consistent with expression of DAZL. DAZL is a conserved RNA-binding protein that regulates germline specification, germ cell differentiation, and reproductive development through post-transcriptional control of mRNA translation. Expression of DAZL is primarily associated with germ cell populations and developing reproductive tissues, making it a widely utilized marker of germline lineage commitment and fertility-associated pathways. The observed band migrates close to the predicted molecular weight of approximately 32 kDa and is consistent with endogenous DAZL expression in human testis tissue. Predicted molecular weight: approximately 32 kDa. Observed molecular weight: approximately 30-35 kDa.

SDS-PAGE analysis of purified, BSA-free DAZL antibody as confirmation of integrity and purity.