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Home >> Antibodies >> DAP5 Antibody / EIF4G2 / Death-associated protein 5

DAP5 Antibody / EIF4G2 / Death-associated protein 5 (RQ7341)

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Image RQ7341 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-87 MG cells with DAP5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-87 MG cells with DAP5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human ThP-1 cells with DAP5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DAP5 antibody.
Western blot testing of 1) human MCF7, 2) human HeLa, 3) monkey COS-7, 4) rat skeletal muscle and 5) mouse skeletal muscle with DAP5 antibody. Expected molecular weight ~97 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P78344
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This DAP5 antibody is available for research use only.
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  • Applications : WB, IF, FACS, Direct ELISA
    Reactivity : Human, Mouse, Rat, Monkey

Description

Eukaryotic translation initiation factor 4 gamma 2, also called DAP-5 (Death-associated protein 5) and p97, is a protein that in humans is encoded by the EIF4G2 gene. Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Application Notes

Optimal dilution of the DAP5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E91-E790) was used as the immunogen for the DAP5 antibody.

Storage

After reconstitution, the DAP5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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