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Home >> Antibodies >> DAP3 Antibody

DAP3 Antibody (R34882)

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  Catalog No Formulation Size Price (USD)  
Image R34882-100UG 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide 100 ug 475
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Western blot testing of HeLa lysate with DAP3 antibody at 3ug/ml. Predicted molecular weight: ~46/41/42 kDa (isoforms 1/2/3).
Western blot of HEK293 lysate overexpressing DAP3 probed with DAP3 antibody (mock transfection in lane 1). Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
Western blot of HeLa lysate (control in left lane, post si-RNA-mediated DAP3 knock-down expresson in right lane) with DAP3 antibody at 1ug/ml. Level of knock-down relative to Actin expression level was determined by RT-PCR. Predicted molecular weight: ~46/41/42kDa (isoforms 1/2/3).
ICC staining of guanidinium thiocyanate-treated HeLa cells before (left) and after (right) si-RNA-mediated DAP3 knock-down using DAP3 antibody at 3ug/ml. Detection by DyLight 488 (green); nuclear DAPI stain (blue).
Immunofluorescent staining of human MCF7 cells with DAP3 antibody at 5ug/ml.
IHC testing of FFPE human brain cortex tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
IHC testing of FFPE human tonsil tissue with DAP3 antibody at 2.5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Goat
Clonality Polyclonal (goat origin)
Isotype Goat Ig
Purity Antigen affinity
UniProt P51398
Gene ID 7818
Localization Cytoplasmic
Applications Western Blot : 3-6ug/ml
IF/ICC : 1-5ug/ml
IHC (FFPE) : 2.5-5ug/ml
ELISA (peptide) LOD : 1:16000
Limitations This DAP3 antibody is available for research use only.
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Description

Additional name(s) for this target protein: Death associated protein 3

Application Notes

Optimal dilution of the DAP3 antibody should be determined by the researcher.

1. This DAP3 antibody will detect isoforms 1, 2 and 3.

Immunogen

Amino acids NPSLLERHCAYL were used as the immunogen for this DAP3 antibody. This sequence is common to isoforms 1, 2 and 3.

Storage

Aliquot and store the DAP3 antibody at -20oC.

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