- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Danre Lmo2 Antibody / Rhombotin 2 (RZ1235)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1235 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q9PTJ3 |
| Localization | Nucleus, Cytoplasm, Cell surface |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This Danre Lmo2 antibody is available for research use only. |
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The Danre Lmo2 antibody targets Lmo2, also known as Rhombotin 2, a LIM-domain transcriptional cofactor essential for hematopoietic specification, vascular development, and early lineage commitment in Danio rerio. Zebrafish, also known as Danio rerio, express lmo2 as one of the earliest markers of hemangioblasts, the shared progenitors of blood and endothelial cell lineages. Lmo2 localizes to the nucleus, where it functions as a non-DNA-binding transcriptional regulator that assembles multi-protein complexes to direct erythroid, endothelial, and hematopoietic stem cell development. Its expression in the intermediate cell mass and lateral plate mesoderm positions Lmo2 as a core component of the genetic circuitry that establishes the vertebrate blood system.
Lmo2 belongs to the LIM-only family of proteins, defined by two tandem LIM domains that mediate protein-protein interactions with transcription factors and chromatin-associated regulators. In zebrafish embryos, lmo2 expression marks hemangioblast territory, vascular precursors, and early erythroid cells before circulation is established. A Danre Lmo2 antibody is suitable for examining nuclear localization in hematopoietic progenitors, endothelial precursors, and mesodermal cell populations undergoing lineage specification during early development.
Functionally, Lmo2 acts as a scaffold that recruits essential transcriptional regulators including Gata1a, Scl/Tal1, Ldb1, and E2A family proteins. These complexes activate genetic programs required for erythroid differentiation, primitive myelopoiesis, and endothelial identity. In zebrafish, Lmo2 is indispensable for the formation of functional blood cells, as lmo2 loss disrupts hemoglobin production, vasculature assembly, and circulation onset. Lmo2 also integrates upstream signaling pathways such as Bmp, Vegf, and Notch to coordinate mesodermal patterning with blood and vascular lineage emergence. Its role extends to later hematopoietic stem cell development, where Lmo2 contributes to early kidney marrow specification and maintenance of multipotent progenitors.
Structurally, zebrafish Lmo2 contains two LIM zinc-binding domains that form interaction interfaces for transcription factors and regulatory proteins. These domains enable Lmo2 to stabilize transcriptional complexes without directly binding DNA, allowing flexible regulation of gene networks controlling blood and endothelial development. Zebrafish lmo2 maps to chromosome 25, with promoter and enhancer regions driving expression in hemangioblast clusters and vascular precursors. Co-localization studies frequently detect Lmo2 alongside markers such as gata1a, scl/tal1, fli1a, ldb1, and kdrl, highlighting its placement at the intersection of hematopoietic and vascular gene networks.
A Danre Lmo2 antibody is suitable for detecting Lmo2 in studies focused on hematopoietic lineage specification, erythroid differentiation, endothelial development, and hemangioblast biology in Danio rerio. Its nuclear expression provides detailed insight into early transcriptional events that initiate blood formation and vascular identity. Researchers use Lmo2 expression patterns to examine defects in blood development, characterize hematopoietic mutants, assess vascular assembly, and evaluate how signaling pathways influence early lineage decisions. These properties make the antibody valuable for research in vertebrate hematopoiesis, vascular development, transcription factor network assembly, and early embryonic patterning. This reagent is supplied for research use by NSJ Bioreagents.
Optimal dilution of the Danre Lmo2 antibody should be determined by the researcher.
E. coli-derived Danre Lmo2 recombinant protein (amino acids M1-V159) was used as the immunogen for the Danre Lmo2 antibody.
After reconstitution, the Danre Lmo2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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