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Home >> Antibodies >> Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody

Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody (R32667)

  Catalog No Formulation Size Price (USD)  
Image R32667 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Cytokeratin 8 Antibody Human Colon Cancer IF. Immunofluorescent staining of FFPE human colon cancer tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a Cy3-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Cytokeratin 8 displays the characteristic cytoplasmic intermediate filament network within the epithelial tumor cells, consistent with its expected localization. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Lung Cancer IF. Immunofluorescent staining of FFPE human lung cancer tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a Cy3-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Strong cytoplasmic staining highlights the characteristic intermediate filament network of epithelial tumor cells, consistent with the expected localization of Cytokeratin 8. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Thyroid Cancer IF. Immunofluorescent staining of FFPE human thyroid cancer tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a DyLight 594-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Cytokeratin 8 staining highlights the cytoplasmic intermediate filament network of the thyroid tumor epithelium, consistent with the expected localization of this epithelial marker. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Ovarian Cancer IF. Immunofluorescent staining of FFPE human ovarian cancer tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a DyLight 594-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Cytokeratin 8 staining highlights the cytoplasmic intermediate filament network of the ovarian tumor epithelium, consistent with the expected localization of this epithelial marker. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Placenta IF. Immunofluorescent staining of FFPE human placenta tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a DyLight 594-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Cytokeratin 8 staining highlights the trophoblast cell layer and its characteristic cytoplasmic intermediate filament network, consistent with the expected localization of this epithelial marker. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Appendix IF. Immunofluorescent staining of FFPE human appendix tissue using Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by detection with a DyLight 594-conjugated secondary antibody (red). Cell nuclei were counterstained with DAPI (blue). Cytokeratin 8 staining highlights the cytoplasmic intermediate filament network within the glandular epithelium, consistent with the expected localization of this epithelial marker. This Cytokeratin 8 Antibody for IF is suitable for studies of epithelial cell morphology, cytoskeletal organization, intermediate filament biology, and protein localization by immunofluorescence microscopy.
Cytokeratin 8 Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue using Cytokeratin 8 Antibody / KRT8 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Strong membranous and cytoplasmic staining is observed throughout the glandular tumor epithelium, consistent with the expected localization of Cytokeratin 8. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, epithelial tumor biology, and Cytokeratin 8 protein localization.
Cytokeratin 8 Antibody Human Thyroid Cancer IHC. Immunohistochemical staining of FFPE human thyroid cancer tissue using Cytokeratin 8 Antibody / KRT8 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Strong membranous and cytoplasmic staining is observed throughout the glandular tumor epithelium, consistent with the expected localization of Cytokeratin 8. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, epithelial tumor biology, and Cytokeratin 8 protein localization.
Cytokeratin 8 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using Cytokeratin 8 Antibody / KRT8 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Strong membranous and cytoplasmic staining is observed throughout the tumor cells, consistent with the expected localization of Cytokeratin 8. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, epithelial tumor biology, and Cytokeratin 8 protein localization.
Cytokeratin 8 Antibody Human Placenta IHC. Immunohistochemical staining of FFPE human placenta tissue using Cytokeratin 8 Antibody / KRT8 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Strong membranous and cytoplasmic staining is observed in placental trophoblasts, consistent with the expected localization of Cytokeratin 8 in epithelial-derived cells. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, placental biology, and Cytokeratin 8 protein localization.
Cytokeratin 8 Antibody Human Kidney Cancer IHC. Immunohistochemical staining of FFPE human kidney cancer tissue using Cytokeratin 8 Antibody / KRT8 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed using pH8 EDTA buffer, followed by HRP-conjugated secondary antibody detection and DAB chromogen. Strong membranous and cytoplasmic staining is observed throughout the tumor cells, consistent with the expected localization of Cytokeratin 8. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, epithelial tumor biology, and Cytokeratin 8 protein localization.
Cytokeratin 8 Antibody Human, Rat and Mouse WB. Western blot analysis of lane 1: human A549, lane 2: human A431, lane 3: human HepG2, lane 4: rat liver, lane 5: rat RH35, lane 6: mouse liver, and lane 7: mouse Hepa1-6 lysates using Cytokeratin 8 Antibody / KRT8 Antibody at 0.5 ug/ml. A specific band was detected at approximately 54 kDa, consistent with the expected molecular weight of Cytokeratin 8, with additional lower molecular weight bands observed in selected samples that may represent processed or tissue-specific forms. This Cytokeratin 8 Antibody, also called KRT8 Antibody, is suitable for studies of epithelial differentiation, intermediate filament organization, epithelial biology, and Cytokeratin 8 protein expression across multiple species.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P05787
Localization Cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody is available for research use only.
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Description

Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody is optimized for visualizing the intracellular distribution of Cytokeratin 8, a type II intermediate filament protein that forms the structural framework of simple epithelial cells. Cytokeratin 8 assembles with type I keratins, primarily Cytokeratin 18, to create filament networks that maintain cell shape, mechanical stability, and epithelial integrity. Immunofluorescence provides a powerful method for examining the characteristic filamentous organization of Cytokeratin 8 while simultaneously evaluating cellular morphology and subcellular localization. NSJ Bioreagents offers Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody for fluorescence microscopy studies of epithelial cells and tissues.

Immunofluorescence staining enables Cytokeratin 8 to be visualized within the cytoplasmic intermediate filament network, allowing researchers to assess cytoskeletal organization, epithelial differentiation, and changes associated with development, injury, or disease. Fluorescent imaging is particularly valuable for investigating filament remodeling during cell migration, mitosis, apoptosis, epithelial-to-mesenchymal transition, and cellular stress. When combined with nuclear stains and additional fluorescent markers, Cytokeratin 8 localization can be correlated with changes in cell architecture and protein expression.

Because Cytokeratin 8 is broadly expressed in simple epithelial tissues and many epithelial-derived tumors, immunofluorescence detection is widely used in cancer research, stem cell biology, organoid studies, and tissue culture models. High-resolution fluorescence microscopy also supports co-localization experiments examining interactions between Cytokeratin 8 and other cytoskeletal proteins, cell junction components, or signaling molecules involved in epithelial homeostasis.

A Cytokeratin 8 Antibody for IF is a valuable research tool for visualizing intermediate filament organization, epithelial cell morphology, cytoskeletal dynamics, protein localization, and epithelial differentiation using immunofluorescence microscopy.

Researchers seeking protein microarray validated detection of Keratin 8 for epithelial differentiation, cytoskeletal biology, and cancer research applications may also be interested in our Cytokeratin 8 Antibody page.

Application Notes

Optimal dilution of the Cytokeratin 8 Antibody for IF / Cytokeratin 8 Immunofluorescence Antibody should be determined by the researcher.

Immunogen

Amino acids D107-K325 from the human protein were used as the immunogen for the Cytokeratin 8 antibody.

Storage

After reconstitution, the Cytokeratin 8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Cytokeratin 8 Antibody for IF, Cytokeratin 8 Immunofluorescence Antibody, KRT8 Antibody for IF, CK8 Antibody for IF

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