Cytokeratin 8 Antibody / Protein Microarray Validated Antibody [clone KRT8/2115] (V7914)

Cytokeratin 8 Antibody Prostate IHC. Immunohistochemistry staining of FFPE human prostate tissue using Cytokeratin 8 Antibody / Protein Microarray Validated Antibody (clone KRT8/2115) demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within glandular epithelial cell populations. The staining pattern is consistent with expression of Keratin 8 (KRT8), a type II intermediate filament protein that serves as a major structural component of simple epithelial tissues. Robust immunoreactivity highlights the epithelial architecture of prostatic glandular structures and supports the established role of KRT8 in maintaining epithelial integrity, cytoskeletal organization, and tissue homeostasis. The observed staining demonstrates the utility of this antibody for studies of epithelial differentiation, intermediate filament biology, prostate tissue organization, and epithelial-derived neoplasia. HIER: boil tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

Cytokeratin 8 Antibody Basal Cell Carcinoma IHC. Immunohistochemistry staining of FFPE human basal cell carcinoma tissue using Cytokeratin 8 Antibody / Protein Microarray Validated Antibody (clone KRT8/2115) demonstrates strong membranous and cytoplasmic HRP-DAB brown staining within nests and cords of neoplastic epithelial cells. The staining pattern is consistent with expression of Keratin 8 (KRT8), a type II intermediate filament protein involved in epithelial cell structure, cytoskeletal organization, and tissue integrity. Prominent immunoreactivity sharply highlights tumor cell populations against the surrounding stromal background and supports the established role of KRT8 as a marker of epithelial differentiation. The observed staining demonstrates the utility of this antibody for studies of epithelial biology, skin cancer pathology, intermediate filament organization, and epithelial-derived neoplasms. HIER: boil tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 minutes and allow to cool before testing.

Cytokeratin 8 Antibody HCT116 WB. Western blot analysis of human HCT116 cell lysate using Cytokeratin 8 Antibody / Protein Microarray Validated Antibody (clone KRT8/2115) demonstrates a prominent immunoreactive band at approximately 56 kDa. The observed molecular weight is consistent with the predicted size of Keratin 8 (KRT8), a type II intermediate filament protein that serves as a major structural component of simple epithelial cells. Detection of the expected target in HCT116 colorectal carcinoma cells is consistent with the established expression of KRT8 in epithelial-derived tissues and malignancies. The observed immunoreactivity demonstrates the utility of this antibody for studies of epithelial differentiation, intermediate filament organization, cytoskeletal biology, tumor cell characterization, and epithelial-derived cancer research. Observed molecular weight: ~56 kDa. Expected molecular weight: ~56 kDa.

Cytokeratin 8 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full-length human proteins using Cytokeratin 8 Antibody / Protein Microarray Validated Antibody (clone KRT8/2115) demonstrates exceptional specificity for Keratin 8 (KRT8). KRT8 was identified as the highest-ranking target with a Z-score of 118.93 and an S-score of 73.45, substantially exceeding all other proteins present on the array. The second-ranked target, KRT5, exhibited a Z-score of 45.48 and an S-score of 9.92, while KRT6A displayed a Z-score of 35.56 and an S-score of 12.46. The Z-score represents the strength of antibody binding relative to the mean signal generated across the array, while the S-score reflects the specificity gap between a target and the next highest-ranking protein. Because antibodies with S-scores greater than 2.5 are generally considered highly specific, the exceptionally high S-score observed for KRT8 provides strong evidence of target selectivity. These results support the utility of clone KRT8/2115 for specific detection of Cytokeratin 8 in epithelial biology, cytoskeletal research, and cancer-related applications.fore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 8 antibody (clone KRT8/2115) as confirmation of integrity and purity.