Cytokeratin 8 Antibody Cocktail H1 + TS1

	Catalog No	Formulation	Size	Price (USD)
	V2172-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2172-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2172SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2172IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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Cytokeratin 8 antibody H1 + TS1 immunohistochemistry colon carcinoma

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IHC testing of human colon carcinoma stained with Cytokeratin 8 antibody cocktail (H1 + TS1).

Immunofluorescent staining of permeabilized human HeLa cells with Cytokeratin 8 antibody cocktail (clones H1 + TS1, green) and Reddot nuclear stain (red).

Immunofluorescent staining of permeabilized human HCT-116 cells with Cytokeratin 8 antibody cocktail (clones H1 + TS1, green) and Reddot nuclear stain (red).

Western blot testing of human HCT-116 cell lysate with Cytokeratin 8 antibody cocktail.

Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 8 antibody cocktail (clones H1 + TS1); Red=isotype control, Blue= Cytokeratin 8 antibody.

IHC testing of human lung carcinoma stained with Cytokeratin 8 antibody cocktail (H1 + TS1).

Species Reactivity	Human, Rat
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	H1 + TS1
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	3856
Localization	Cytoplasmic
Applications	Flow cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western blot : 1-2ug/ml
Limitations	This Cytokeratin 8 antibody is available for research use only.
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Description

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular (ï¿½ring-like, perinuclearï¿½) from ductal (ï¿½peripheral-predominantï¿½) carcinoma of the breast.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A cytoskeleton preparation containing Cytokeratin 8 (H1) and Keratin preparation from a human carcinoma (TS1) were used as the immunogen for this antibody cocktail.