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Home >> Antibodies >> Cytokeratin 8/18 Antibody Cocktail

Cytokeratin 8/18 Antibody Cocktail [clone SPM192 + SPM265] (V8817)

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  Catalog No Formulation Size Price (USD)  
Image V8817-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V8817-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V8817SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human lung carcinoma tissue with Cytokeratin 8/18 antibody (clone SPM192 + SPM265). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma tissue with Cytokeratin 8/18 antibody (clone SPM192 + SPM265). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM192 + SPM265
Purity Protein A/G affinity
UniProt P05787 , P05783
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Cytokeratin 8/18 antibody is available for research use only.
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Description

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Application Notes

Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.

Immunogen

Keratin preparation from a human carcinoma (SPM192); PMC-42 human breast carcinoma cells (SPM265) was used as the immunogen for the Cytokeratin 8/18 antibody.

Storage

Aliquot the Cytokeratin 8/18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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