- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 7 Antibody [clone OV-TL12/30] (V2654BTN)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V2654BTN | 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 500 ul | 519 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Biotin Conjugate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | OV-TL12/30 |
| Purity | Protein G affinity chromatography |
| UniProt | P08729 |
| Localization | Cytoplasmic |
| Applications | Flow cytometry : 5ul per test per one million cells or 5ul per 100ul of whole blood Immunofluorescence : 2-4ug/ml Western blot : 2-4ug/ml Immunohistochemistry (FFPE) : 2-4ug/ml at RT |
| Limitations | This Cytokeratin 7 antibody is available for research use only. |
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It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.
The ovarian carcinoma cell line OTN 11 was used as the immunogen for the Cytokeratin 7 antibody.
Store the Cytokeratin 7 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
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