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Home >> Antibodies >> Cytokeratin 7 Antibody KRT7/3733 / Mouse Monoclonal

Cytokeratin 7 Antibody KRT7/3733 / Mouse Monoclonal [clone KRT7/3733] (V4278)

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  Catalog No Formulation Size Price (USD)  
Image V4278-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V4278-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V4278SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Western blot testing of human SK-BR-3 cell lysate with Cytokeratin 7 Antibody KRT7/3733.
IHC staining of FFPE human bladder carcinoma with Cytokeratin 7 Antibody KRT7/3733. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with Cytokeratin-7 antibody (clone KRT7/3733). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin-7 antibody (clone KRT7/3733) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name KRT7/3733
Purity Protein A/G affinity
UniProt P08729
Localization Cytoplasm
Applications Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This Cytokeratin 7 Antibody KRT7/3733 is available for research use only.
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Description

Cytokeratin 7 Antibody KRT7/3733 recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. mAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

For an alternative antibody targeting the same protein, see our Cytokeratin 7 Antibody OV-TL12/30 (V2654), a mouse monoclonal antibody validated for WB, IHC, IF, and flow cytometry.

Application Notes

Optimal dilution of the Cytokeratin 7 Antibody KRT7/3733 should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Cytokeratin 7 antibody.

Storage

Aliquot the Cytokeratin 7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Keratin 7 antibody, CK7 antibody, CK-7 antibody, KRT7 antibody, Cytokeratin-7 antibody, Keratin Type II Cytoskeletal 7 antibody, Type II Cytokeratin 7 antibody, Cytokeratin 7/CK7 antibody, K7 antibody

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