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Home >> Antibodies >> Cytokeratin 7 Antibody / KRT7

Cytokeratin 7 Antibody / KRT7 [clone KRT7/2200] (V7913)

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  Catalog No Formulation Size Price (USD)  
Image V7913-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7913-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7913SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human lung carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with Cytokeratin 7 antibody (clone KRT7/2200). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa cell lysate with Cytokeratin 8 antibody (clone KRT7/2200). Predicted molecular weight ~51 kDa.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 17 antibody (clone KRT7/2200) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name KRT7/2200
Purity Protein G affinity chromatography
UniProt P08729
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Cytokeratin 7 antibody is available for research use only.
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Description

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. MAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Application Notes

Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

Immunogen

A recombinant full-length human KRT7 protein was used as the immunogen for this Cytokeratin 7 antibody.

Storage

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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