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Home >> Antibodies >> Cytokeratin 7 Antibody / Keratin 7

Cytokeratin 7 Antibody / Keratin 7 [clone CTKN7-1] (V7104)

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  Catalog No Formulation Size Price (USD)  
Image V7104-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7104-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7104SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7104IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone CTKN7-1, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (clone CTKN7-1, green) and Reddot nuclear stain (red).
IHC testing of FFPE human lung carcinoma and Cytokeratin 7 antibody (clone CTKN7-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
IHC testing of human ovarian carcinoma and Cytokeratin 7 antibody (clone CTKN7-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone CTKN7-1). Predicted molecular weight ~51 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CTKN7-1
Purity Protein G affinity chromatography
UniProt P08729
Localization Cytoplasmic
Applications Western Blot : 1-2ug/ml
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC Only Format : incubate for 30 min at RT (1)
Limitations This Cytokeratin 7 antibody is available for research use only.
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Description

Cytokeratin 7 antibody targets Cytokeratin 7, also known as Keratin 7, a type II intermediate filament protein that is a core structural component of simple and glandular epithelial cells. Cytokeratin 7 is a cytoplasmic protein that contributes to epithelial integrity by forming heteropolymeric filaments with type I keratins, supporting cellular architecture, polarity, and mechanical stability. Because of its selective expression pattern, Cytokeratin 7 antibody is widely used in epithelial biology and cancer research to characterize epithelial lineage and differentiation status.

Cytokeratin 7 expression is prominent in a broad range of glandular and ductal epithelia, including those of the lung, breast, biliary tract, pancreas, endometrium, ovary, and urinary tract. In contrast, normal colorectal epithelium and most stratified squamous epithelia lack Cytokeratin 7 expression. This well-defined distribution has established CK7 as a key epithelial marker for distinguishing tissue origin and cellular phenotype in complex samples. As a result, Cytokeratin 7 antibody detection is frequently applied in studies examining epithelial organization and lineage specification.

In epithelial-derived cancers, Cytokeratin 7 expression reflects underlying differentiation programs and tumor phenotype. CK7-positive staining is commonly observed in carcinomas of the lung, breast, ovary, endometrium, biliary system, and urothelium, while many gastrointestinal adenocarcinomas remain CK7 negative. These expression patterns provide valuable biological context when evaluating epithelial tumors and studying mechanisms of epithelial transformation and tumor heterogeneity.

Beyond its diagnostic relevance, Cytokeratin 7 participates in dynamic cytoskeletal remodeling during epithelial development, regeneration, and disease-associated stress responses. Changes in Keratin 7 expression often accompany epithelial plasticity, glandular metaplasia, and malignant progression. Antibodies recognizing Cytokeratin 7 therefore serve as reliable tools for investigating epithelial differentiation, tissue architecture, and disease-related alterations in cytoskeletal composition across diverse research applications.

Application Notes

Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.

Storage

Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

   1.   View all Cytokeratin 7 antibodies

   2.   View all Keratin antibodies

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