CK6 Antibody SPM269 / Keratin 6 Monoclonal Antibody [clone SPM269] (V9037)

Description

CK6 Antibody SPM269 recognizes cytokeratin 6, a member of the type II intermediate filament protein family involved in epithelial differentiation, mechanical stability, and responses to tissue injury. Keratin 6 proteins are encoded by three closely related genes, KRT6A, KRT6B, and KRT6C, located on chromosome 12. These proteins share substantial sequence similarity and are expressed in selected stratified epithelia, including hair follicles, nail beds, and specialized mucosal surfaces. Keratin 6 expression can also increase in activated keratinocytes during wound healing and inflammatory conditions, making it an important marker of epithelial remodeling.

Keratin 6 proteins assemble into intermediate filaments through interactions with type I keratins, particularly keratins 16 and 17. These filament networks contribute to cytoskeletal organization and help epithelial cells adapt to mechanical and physiological stress. Unlike keratin 5, which is predominantly associated with basal epithelial cells, keratin 6 is frequently induced during keratinocyte activation and hyperproliferation. Its expression is regulated by differentiation signals, inflammatory mediators, and tissue injury. This dynamic regulation makes CK6 useful for investigating epidermal repair, epithelial maturation, and pathological changes in keratinocyte behavior.

CK6 is widely studied in squamous cell carcinoma, where its expression can help characterize squamous differentiation. It is frequently evaluated alongside KRT5 and p63 in studies of epithelial tumor identity. Increased keratin 6 expression is also associated with hyperproliferative skin disorders, including psoriasis, and with epithelial responses to chronic inflammation. Mutations in KRT6A and KRT6B can cause pachyonychia congenita, an inherited disorder characterized by nail dystrophy, painful palmoplantar keratoderma, and other abnormalities of keratinized epithelia.

NSJ Bioreagents offers mouse monoclonal clone SPM269, developed against a C-terminal peptide sequence of human keratin 6. The antibody is reported to recognize human and mouse samples and is supported by immunohistochemistry, immunofluorescence, and flow cytometry data. Because keratin 6 isoforms are highly homologous, recognition of individual family members should not be assumed without isoform-specific validation. The Keratin 6 Monoclonal Antibody provides an additional clone option for investigating epithelial differentiation and keratinocyte activation. A CK6 Antibody SPM269 supports research into squamous epithelial biology, tissue remodeling, and keratin-associated disease.

Explore our protein microarray-validated Cytokeratin 6 Antibody KRT6A/2368 for investigating keratinocyte activation, squamous differentiation, and KRT6A expression in epithelial tissues.

Application Notes

The optimal dilution of the CK6 Antibody SPM269 / Keratin 6 Monoclonal Antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids GSSTIKYTTTS from the C-terminus of human keratin 6 was used as the immunogen for this CK6 Antibody SPM269.

Storage

Store the CK6 Antibody SPM269 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cytokeratin 6 antibody, Keratin 6 antibody, KRT6 antibody, Cytokeratin-6 antibody, CK-6 antibody, Type II Cytoskeletal Keratin 6 antibody