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Home >> Antibodies >> Cytokeratin 5/6/18 Antibody

Cytokeratin 5/6/18 Antibody [clone LP34] (V7633)

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  Catalog No Formulation Size Price (USD)  
Image V7633-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7633-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7633SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Cytokeratin 5/6/18 Antibody Human Skin Tissue IHC. Immunohistochemistry staining of FFPE human skin with Cytokeratin 5/6/18 antibody clone LP34. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 5/6/18 antibody clone LP34 as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name LP34
Purity Protein G affinity chromatography
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.1-0.2ug/ml
Limitations This Cytokeratin 5/6/18 antibody is available for research use only.
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Description

Cytokeratin 5/6/18 Antibody clone LP34 detects Cytokeratin 5, Cytokeratin 6 and Cytokeratin 18, polypeptides of 58 kDa, 56 kDa, and 45 kDa, respectively, also called KRT5, KRT66, and KRT18. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

This antibody is part of a broader Cytokeratin antibody panel, and additional KRT family antibodies can be explored on the Cytokeratin antibody landing page.

Application Notes

Optimal dilution of the Cytokeratin 5/6/18 antibody clone LP34 should be determined by the researcher.

Immunogen

The detergent-insoluble fraction of psoriatic human epidermis was used as the immunogen for the Cytokeratin 5/6/18 antibody.

Storage

Store the Cytokeratin 5/6/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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